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Briefly, viral RNA was isolated from 140 μl of patient serum using QiAmp Viral RNA isolation system (Qiagen, Germany) and 5 μl of the RNA was RT-PCR amplified using single step RT-PCR kit (USB, Cleveland, Ohio) using D1F and DencomR2 primers for initial virus detection, or using D1F and nTS1/nTS2/nTS3/nDen4 primers (Table (Table2) 2) for detection of the serotype. The Vigilance and Anti-Corruption Bureau have recorded the arrest of VK Ebrahim Kunju, a minister in Kerala's former ruling dispensation, United Democratic Front … Replies. Reply Delete. Meen valarpu tamilnadu. Anglais. Udumalai. Please contact.. Last edited by a moderator: Jan 7, 2011. Like Like. katla. In the dataset comprising of all genotype strains (N = 58), three codons were found to be under positive selection pressure by the REL method i.e. 01. Tamoul. VIRAL IN NZ. Drip and Fertigation system Subsidy cost / acre 32,000 4. Thread starter sureshsamuel; Start date Dec 30, 2010; S. sureshsamuel Member. Tamoul. Anglais . 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